Stronger bones thanks to heat and microbiota

Osteoporosis, a bone disease linked to aging, is characterized by a loss of bone density, micro-architectural deterioration of the bones, and an increased risk of fractures. With one-third of postmenopausal women affected, it is a major public health problem. Through epidemiological analyses, laboratory experiments, and state-of-the-art metagenomic and metabolomics tools, a research team from the University of Geneva (UNIGE), in Switzerland, has observed that exposure to warmer ambient temperatures (93.2 °F) increases bone strength while preventing the loss of bone density typical of osteoporosis. Moreover, this phenomenon, linked to a change in the composition of gut microbiota triggered by heat, could be replicated by transplanting the microbiota of mice living in a warm environment to mice suffering from osteoporosis. Indeed, after the transplant, their bones were stronger and denser. These results, to be discovered in Cell Metabolism, make it possible to imagine effective and innovative interventions to prevent and treat osteoporosis.

Many biologists are familiar with Allen’s Rule, from 19th-century naturalist Joel Asaph Allen, according to which animals living in warm areas have a larger surface area about their volume than animals living in colder environments. Indeed, a larger skin surface allows better evacuation of body heat. “In one experiment, we placed newborn mice at a temperature of 34 °C to minimize the heat shock associated with their birth. We found that they had longer and stronger bones, confirming that bone growth is affected by ambient temperature,” explains Mirko Trajkovski, Professor at the Department of Cell Physiology and Metabolism and at the Diabetes Centre of the UNIGE Faculty of Medicine, who led the study. But what about adulthood?

Consistent epidemiological data

By placing several groups of adult mice in a warm environment, the scientists observed that bone size remained unchanged while bone strength and density were largely improved. They then repeated their experiment with mice after an ovariectomy modeling post-menauposal osteoporosis. “The effect was very interesting,” says Claire Chevalier, then a researcher in Professor Trajkovski’s laboratory and the first author of this work. “The simple fact of warming the living environment of our mice protected them from the bone loss typical of osteoporosis!”

What about human beings? The research team analyzed global epidemiological data on osteoporosis incidence about the average temperature, latitude, calcium consumption, and vitamin D levels. Interestingly, they found that the higher the temperature, the fewer hip fractures — one of the main consequences of osteoporosis — regardless of other factors. “We found a clear correlation between geographical latitude and hip fractures, meaning that the incidence is higher in the northern countries than the warmer south,” says Mirko Trajkovski. “Normalising, the known players' analysis, such as vitamin D or calcium, did not modify this correlation. However, when we excluded the temperature as the determinant, the correlation was lost. This is not to say that calcium or vitamin D does not play a role, either alone or in combination. However, the determining factor is heat -or lack thereof.”

How the microbiota adapts

Specialists in the microbiota, the Geneva scientists, wanted to understand its role in these metabolic modifications. To this end, they transplanted the microbiota of mice living in a 34° environment to osteoporotic mice whose bone quality was rapidly improved. “These findings may imply an extension to Allen’s rule, suggesting elongation-independent effects of the warmth, which predominantly favors bone density and strength during adulthood through microbiota alterations,” says Mirko Trajkovski.

Thanks to the state-of-the-art metagenomic tools developed in their laboratory, the scientists then understood the role of microbiota. When adapts to heat, it leads to a disruption in the synthesis and degradation of polyamines, molecules involved in aging, and bone health. “With heat, the synthesis of polyamines increases, while their degradation is reduced. They thus affect osteoblasts' activity (the cells that build bones) and reduce the number of osteoclasts (the cells that degrade bones). With age and menopause, the exquisite balance between the osteoclast and osteoblast activity is disrupted,” explains Claire Chevalier. “However, heat, by acting on the polyamines, which we found to be partly regulated by the microbiota, can maintain the balance between these two cell groups.” These data, therefore, indicate that exposure to warmth could be a prevention strategy against osteoporosis.

Developing new treatments

The influence of microbiota on metabolism is being better understood. However, to use this knowledge to develop therapeutic strategies, scientists must identify precisely the role of particular bacteria in particular diseases. In the context of their work on osteoporosis, Professor Trajkovski’s team has been able to identify certain important bacteria. “We still need to refine our analyses, but our relatively short-term goal would be to identify candidate bacteria and develop several ‘bacterial cocktails’ to treat metabolic and bone disorders, such as osteoporosis, but also to improve insulin sensitivity, for example,” the authors conclude.

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Materials provided by Université de Genève.

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